Non particular binding was Proposed hypotheses blocked by incubat

Non particular binding was Proposed hypotheses blocked by incubation with 5% bovine serum albumin in Tris buffered saline Tween 20 at 22 C, then membranes had been incubated overnight or for 36 forty hr at four C with rabbit anti phospho ERK 44 42, mouse anti total ERK 44 42, rabbit anti MKP 1, goat

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The blots have been then incubated with horseradish peroxidase go

The blots were then incubated with horseradish peroxidase goat anti rabbit or anti mouse IgG for 1 h. Signal was detected by utilizing an enhanced chemi luminescence industrial kit. The relative photographic density was quantitated by scanning the photographic negatives on the gel docu mentation and analysis program. Statistical evaluation Statistically important differences have been

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As pointed out above, after the X ray complexes have been superim

As outlined above, just after the X ray complexes had been superimposed, some dif ferences emerged in the side chain conformations of Thr231, Thr232 and Gln73, which indicates that these residues can move and rotate, using the most notable movement happening for Gln73, to superior accommodate the inhibitors. Additionally, some expected flexibility was also observed

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