For glycogen synthase analysis, the lysates were prepared in buffer containing of 50mMTris HCl, 10 mM EDTA, 10 mM NaCl, 50 mM NaF, 1 mM microcystin LR, 1% Nonidet P 40 and 1% protease inhibitor cocktail. The cells were scraped, collected within an eppendorf and permitted to stand on ice for 30 min. The lysates
(Read More…)
Antibodies against Mdm2, estrogen receptor alpha, p53, Bax, p73, alpha fetoprotein, cyclin D1, caveolin 1, Akt, pAkt, W tubulin, and T actin were ordered from Santa Cruz Biotechnology, CA, USA. Antibody certain to phospho caveolin was obtained from BD Bioscience, CA, USA. Human breast cancer cell lines MCF 7, MDAMB231, and MDA MB 468 were
(Read More…)
Transfection with wild typ-e c Abl triggered reduced expression of c Abl and Shb compared to transfection with kinase inactive c Abl. Despite the reduction in the total Shb information, Shb tyrosine phosphorylation remained unchanged after transfection with wild typ-e h Abl and moved with reduced mobility, indicating a heightened relative Shb tyrosine phosphorylation involving
(Read More…)
Follow @selleckchem on twitter.
